Journal: Oncogenesis

Article Title: Endothelial Caveolin-1 regulates the radiation response of epithelial prostate tumors

doi: 10.1038/oncsis.2015.9

Figure Lengend Snippet: Tumor growth and tumor response to radiation therapy critically depend on endothelial Cav1 expression. Syngeneic MPR xenografts implanted into Cav1-deficient C57Bl/6 mice grew faster and displayed less integration of smooth muscle cells into the wall of newly formed blood vessels indicative for a less stabilized vessel phenotype compared with tumors from Cav1 wild-type animals. High Cav1 expression in ECs protected vascular structures from radiation-induced damage at a clinically relevant dose, resulting in a decreased tumor growth delay upon irradiation. In contrast, the loss of stromal Cav1 increased the sensitivity of ECs to radiation-induced apoptosis thereby enhancing tumor growth delay upon radiotherapy. Thus, Cav1 content of vascular cells determines the sensitivity to microvascular damage and is critical for the regulation of the tumor response to radiation. Thus, the pro-survival factor Cav1 might be a promising therapeutic target for combinatorial therapies to counteract radiation resistance of prostate cancer at the level of the tumor vasculature.

Article Snippet: The human microvascular endothelial cell line AS-M5 was cultured in ECG medium (PromoCell).

Techniques: Expressing, Irradiation

Journal: The Open Biomedical Engineering Journal

Article Title: Interactions of Human Endothelial and Multipotent Mesenchymal Stem Cells in Cocultures

doi: 10.2174/1874120701004010190

Figure Lengend Snippet: The proliferation rate of hMSC is donor dependent. Cumulative population doublings of hMSC from 3 different donors ( A , B , C ) and AS-M.5 ( D ) in cultures supplemented with M1 (diamonds, full line), M3 (triangles, dashed), and M2 (squares, dashed line) in the course of 21 days of cultivation. Indicated are means +/- SEM.

Article Snippet: Endothelial cell line AS-M.5 cells [ , ] were propagated in Endothelial Cell Growth Medium MV (PromoCell, Heidelberg, Germany).

Techniques:

Journal: The Open Biomedical Engineering Journal

Article Title: Interactions of Human Endothelial and Multipotent Mesenchymal Stem Cells in Cocultures

doi: 10.2174/1874120701004010190

Figure Lengend Snippet: Cell culture media affect the morphology of hMSC. The hMSC ( A , B , C ) and AS-M.5 ( D , E , F ) were grown in medium M1 ( A , D ), medium M2 ( B , E ), and medium M3 ( C , F ) for 18 days. Phase contrast, bar represents 100 µm.

Article Snippet: Endothelial cell line AS-M.5 cells [ , ] were propagated in Endothelial Cell Growth Medium MV (PromoCell, Heidelberg, Germany).

Techniques: Cell Culture

Journal: The Open Biomedical Engineering Journal

Article Title: Interactions of Human Endothelial and Multipotent Mesenchymal Stem Cells in Cocultures

doi: 10.2174/1874120701004010190

Figure Lengend Snippet: Appearance of the cocultures of hMSC and EC. Mixtures of EGFP-transduced hMSC and AS-M.5 were cultured in M2 medium for 21 days. CD31-positive endothelial cells were detected by immunofluorescence. In cocultures ( A ), both cell typed developed distinct clusters containing either endothelial cells ( B ) or hMSC ( C ). Close cell contact of hMSC and EC were observed only sporadically ( D , E ). Bar represent 100µm (A), 20 µm ( B - E ).

Article Snippet: Endothelial cell line AS-M.5 cells [ , ] were propagated in Endothelial Cell Growth Medium MV (PromoCell, Heidelberg, Germany).

Techniques: Cell Culture, Immunofluorescence